PERTUMBUHAN OKULASI JERUK KEPROK BRASTEPU (Citrus nobilis Var. Brastepu) MENGGUNAKAN JERUK ASAM SEBAGAI BA TANG BA WAH

Authors

  • Isnaini Nurwahyuni
  • Justin A Napitupulu
  • Rosmayati .
  • Fauziah Harahap

Keywords:

okulasi, jeruk keprok, Citrus nobilis, CVPD, Isolasi DNA, PCR.

Abstract

The growth of Citrus nobilis Var. Brastepu budding that is free fromCitrus Vein Phloem Degeneration (CVPD) is explained. The method is conductedby taking of bud stick from a selected healthy citrus Brastepu plant and is thengrafted into a sour orange (Citrus auraniium) as mother trees via budding method.The research is conduted in Desa Bukit, Brastagi North Sumatra, Indonesia.Optimum conditions on the budding technique have been obtained to produce goodqualih; seedling of Citrus nobilis Var. Brastepu. Growth characteristics of the plantin various treatment conditions have been studied based on the growth leaves, stem,and branches. Analysis of the DNA using RAPD-PCR method has confirmed thatall new seedling produced in the propagation method are free from CVPD.

References

Almeida, R.P.P., Nascimento, F.E., Chau, J.,

Prado, S.S., Tsai, CW, Lopes, S.A.,

dan Lopes, J.R.S., (2008), Genetic

Structure and Biology of Xylella

fastidiosa Strains Causing Disease in

Citrus and Coffee in Brazil, Applied

And Environmental Microbiology, 74

(12), 3690-3701.

Bove, J.M., 2006. Huanglongbing: A

destructive, newly-emerging,

century old disease of citrus.

Journal of Plant Pathology 88(1): 7-

Do Carmo, T.D; Luc,

Cristina, M.E.;

Takao, Y.P.;

D.J.; Eveillard, S.;

de Jesus, J.WC.;

Aparecido, L.S.;

Beozzo, B.R.; Juliano, A.A.; Saillard,

C.; Bove, JM., (2005), Citrus

huanglongbing in Sao Paulo State,

Brazil: PCR detection of the

'Candidatus' Liberibacter species

associated with the disease, Mol

Cell Probes. 19(3): 173-179.

Kim, J.S., dan Wang, N., (2009)

Characteristization of copy numbers of 16 S DNA and 16 S RNA of

Candidatus Liberibacter asiaticus

and the implication in detection in

planta using quantitative PCR. BMC

Research Notes 2: 37-40.

Prastowo, N.R.; Roshetso, J.M.; Manurung,

G.E.S.; Nugraha, E.; Julian, J.M. dan

Harum, F., (2006), Teknik

pembibitan dan perbanyakan

vegetatif tanaman buah. World

Agroforestry centre (ICRAF). Bogor.

p.87.

Rieger, M., (2006), Introduction to Fruit

Crops. Hawthorn Food Products

Press, NY. p.462.

Sanford M.T. 1992. Pollination of citrus by

honey bees. UF/IFAS document

RF AA092. Available online.

Su, H.J. (2008), Production and cultivation

of virus free citrus saplings for

citrus rehabilitation in Taiwan.,

APCoAB. New Delhi. India.

Sumaatrnaja, G., (2001), Teknik

perbanyakan tanaman jeruk manis

di Kabupaten Bengkulu Selatan.

Laporan Teknis PUSLIT-Biologi-

LIPI. Jakarta.

Williamson, J.G., dan Jackson, L.K., (1994),

Citrus propagation. University of

Florida, Fact Sheet HS-86, June 1994.

http://ultimatecitrus.com

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Published

2018-10-08

Issue

Section

VOL 12, NO 1 (2012): JURNAL PENELITIAN SAINTIKA