The antimicrobial study of white turmeric (Curcuma zedoaria) extracted using deep euthectic solvent (DES) and ultrasonication
DOI:
https://doi.org/10.24114/jpkim.v15i3.49349Abstract
This research is prompted by the emergence of various cases of infectious diseases and antibiotic resistance that often occur and the development of the latest research methods in the field of medicinal plants. One of the medicinal plants that is often used is Curcuma zedoaria which is generally extracted using conventional solvents. This research aims to find new innovations in the extraction process of medicinal plants. This research is a laboratory experiment carried out in vitro. This research uses the latest solvent, called DES (Deep Eutectic Solvent) with the help of ultrasonication to extract active compounds from C. zedoaria as a natural anti-bacterial. DES is known as an environmentally friendly solvent, cheap, easy to make, and is still relatively new in Indonesia. The C. zedoaria extraction process uses two types of DES solvent composition assisted by a ultrasonicator. The research results show that: White turmeric extract (C. zedoaria) obtained by extraction using DES solvent and ultrasonication. Ultrasonication results using 30%, 70% and 100% DES solvent showed antimicrobial properties against Escherichia coli with a lower zone of inhibition compared to the antibiotic ciprofloxacin. Ultrasonication results using 30%, 70% and 100% DES solvent showed antimicrobial properties against Staphylococcus aureus with a lower zone of inhibition compared to ciprofloxacin. TLC data shows that the white turmeric extract obtanined contains various antimicrobial substances which allows to inhibit each other to such an extent that their activity is lower than ciprofloxacin.Keywords: C. zedoaria; Deep eutectic solvent; Escherichia coli; Staphylococcus aureus; UltrasonicationReferences
Adamczak, A., Ożarowski, M., & Karpiński, T. M. (2020). Curcumin, a natural antimicrobial agent with strain-specific activity. Pharmaceuticals, 13(7), 153. https://doi.org/10.3390/ph13070153
Bakirtzi, C., Triantafyllidou, K., & Makris, D. P. (2016). Novel lactic acid-based natural deep eutectic solvents: Efficiency in the ultrasound-assisted extraction of antioxidant polyphenols from common native Greek medicinal plants. Journal of Applied Research on Medicinal and Aromatic Plants, 3(3), 120“127. https://doi.org/10.1016/j.jarmap.2016.03.003
Busman, B., Edrizal, E., & Wirahmi, S. D. (2019). Daya hambat ekstrak rimpang temu putih (Curcuma zedoaria) terhadap Streptococcus mutans dan Staphylococcus aureus. Menara Ilmu, 13(6), 19-28.
Candani, D., Ulfah, M., Noviana, W., & Zainul, R. (2018). A review pemanfaatan teknologi sonikasi. FMIPA Universitas Negeri Padang, Padang.
Das, K., & Rahman, M. A. (2012). Analgesic and antimicrobial activities of Curcuma zedoaria. Int J Pharm Sci, 4(Suppl 5), 322-328.
Dosoky, N., & Setzer, W. (2018). Chemical composition and biological activities of essential oils of curcuma species. Nutrients, 10(9), 1196. https://doi.org/10.3390/nu10091196
Endarini, L.H. (2016). Farmakognisi dan fitokimia, pusdik SDM kesehatan. Jakarta: Kementerian Kesehatan Republik Indonesia.
Gharge, S., Hiremath, S. I., Kagawad, P., Jivaje, K., Palled, M. S., & Suryawanshi, S. S. (2021). Curcuma zedoaria Rosc (Zingiberaceae): a review on its chemical, pharmacological and biological activities. Future Journal of Pharmaceutical Sciences, 7(1). https://doi.org/10.1186/s43094-021-00316-1
Indriani, V., Chiuman, L., Wijaya, L. L., Lister, G., & Grandis, L. (2020). Antibacterial effect of Curcuma zedoaria Extract on Bacillus cereus and Staphylococcus epidermidis. Althea Medical Journal, 7(1), 6“10. https://doi.org/10.15850/amj.v7n1.1886
Julianto, T. S. (2019). Fitokimia tinjauan metabolit sekunder dan skrining fitokimia. Journal of Chemical Information and Modeling, 53, 1-116.
Kautsari, S. N., Purwakusumah, E. D., & Nurcholis, W. (2021). Profil kromatografi lapis tipis ekstrak kunyit (Curcuma longa Linn) segar dan simplisia dengan variasi metode ekstraksi. Media Farmasi, 16(1), 65. https://doi.org/10.32382/mf.v16i1.1403
Kunarto, B., Sutardi, S., Supriyanto, S., & Anwar, C. (2019). Optimasi ekstraksi berbantu gelombang ultrasonik pada biji melinjo kerikil (Gnetum gnemon L., ˜Kerikil™) menggunakan response surface methodology. Jurnal Aplikasi Teknologi Pangan, 8(3), 104. https://doi.org/10.17728/jatp.5122
KurtulbaÅŸ, E., Pekel, A. G., Bilgin, M., Makris, D. P., & Åžahin, S. (2020). Citric acid-based deep eutectic solvent for the anthocyanin recovery from Hibiscus sabdariffa through microwave-assisted extraction. Biomass Conversion and Biorefinery, 12(2), 351“360. https://doi.org/10.1007/s13399-020-00606-3
Kim, K. M., Lee, J. Y., Jeon, B. H., Quan, K. T., Na, M., Nam, K.-W., & Chae, S. (2021). Extract of Curcuma zedoaria R. prevents atherosclerosis in apolipoprotein E-deficient mice. Nutrition Research and Practice, 15(3), 319. https://doi.org/10.4162/nrp.2021.15.3.319
Pacheco-Fernández, I., & Pino, V. (2019). Green solvents in analytical chemistry. Current Opinion in Green and Sustainable Chemistry, 18, 42“50. https://doi.org/10.1016/j.cogsc.2018.12.010
Pangestika, I. I. J., Widayati, A., Hartati, E. L. K. N., & Yugatama, A. (2020). Aktivitas antibakteri pada rimpang famili Zingiberaceae terhadap Staphylococcus aureus dan Escherichia coli. Proceedings National Conference PKM Center, 1, 144-149.
Qin, H., Hu, X., Wang, J., Cheng, H., Chen, L., & Qi, Z. (2020). Overview of acidic deep eutectic solvents on synthesis, properties and applications. Green Energy & Environment, 5(1), 8“21. https://doi.org/10.1016/j.gee.2019.03.002
Rachmaniah, O., Istiqomah, I., Fakhrinanda, F., & Rachimoellah, M. (2020). Central composite design based statistical modelling of curcuminoids extraction from Curcuma zedoaria using choline chloride based Natural Deep Eutectic Solvent (NADES). IOP Conference Series: Materials Science and Engineering, 823(1), 012011. https://doi.org/10.1088/1757-899x/823/1/012011
Teow, S.-Y., Liew, K., Ali, S. A., Khoo, A. S.-B., & Peh, S.-C. (2016). Antibacterial action of curcumin against Staphylococcus aureus: A brief review. Journal of Tropical Medicine, 2016, 1“10. https://doi.org/10.1155/2016/2853045
Silalahi, M. (2020). Curcuma zedoaria (Christm.) roscoe (benefits and bioactivity). Eureka Herba Indonesia, 1(2), 44“52. https://doi.org/10.37275/ehi.v1i2.10
Subositi, D., & Wahyono, S. (2019). Study of the genus Curcuma in Indonesia used as traditional herbal medicines. Biodiversitas Journal of Biological Diversity, 20(5). https://doi.org/10.13057/biodiv/d200527
Tjiptoningsih, U. G. (2021). Uji daya hambat air perasan buah lemon (Citrus limon (L.) BURM. F.) terhadap pertumbuhan bakteri aggregatibacter actinomycetemcomitans. Jurnal Ilmiah dan Teknologi Kedokteran Gigi, 16(2), 86“96. https://doi.org/10.32509/jitekgi.v16i2.1100
Zainal-Abidin, M. H., Hayyan, M., Hayyan, A., & Jayakumar, N. S. (2017). New horizons in the extraction of bioactive compounds using deep eutectic solvents: A review. Analytica Chimica Acta, 979, 1“23. https://doi.org/10.1016/j.aca.2017.05.012
Downloads
Published
Issue
Section
License
Copyright (c) 2023 Timotius Agung Soripada, Erny Tandanu, Refi Ikhtiari
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors published in this journal agree to the following terms:
- The copyright of each article is retained by the author (s).
- The author grants the journal the first publication rights with the work simultaneously licensed under the Creative Commons Attribution License, allowing others to share the work with an acknowledgment of authorship and the initial publication in this journal.
- Authors may enter into separate additional contractual agreements for the non-exclusive distribution of published journal versions of the work (for example, posting them to institutional repositories or publishing them in a book), with acknowledgment of their initial publication in this journal.
- Authors are permitted and encouraged to post their work online (For example in the Institutional Repository or on their website) before and during the submission process, as this can lead to productive exchanges, as well as earlier and larger citations of published work.
Jurnal Pendidikan Kimia is licensed under a Creative Commons Attribution 4.0 International License
.png)
