Determination of phenolic contents and antioxidant activity test of ethanol extract of Sirih merah (Piper crocatum Ruiz & Pav.) leaves using the DPPH method
DOI:
https://doi.org/10.24114/jpkim.v13i2.26984Abstract
The research objectives were to identify the secondary metabolite components, total phenolic content and determine the antioxidant activity of the ethanol extract of red betel leaf (Piper crocatum Ruiz & Pav.). The extraction process was carried out by materation using ethanol as a solvent. Determination of total phenolic content was carried out colorimetrically with Folin-Ciocalteu reagent measured at a maximum wavelength of 765 nm. Determination of antioxidant activity using the DPPH method measured by spectrophotometry at a maximum wavelength of 517 nm. The results of phytochemical screening of the ethanolic extract of red betel leaf contain secondary metabolites, including flavonoid, phenolic, tannin, alkaloids, steroids, and triterpenoids. The total phenolic content of the red betel leaf ethanol extract was 0.949±0.003 mg GAE/g d.w. and has antioxidant activity (IC50) 84,656 including strong category as an antioxidant. Keywords: Piper crocatum Ruiz & Pav., Antioxidant, Ethanol extract, Folin-Ciocalteu and DPPHReferences
Akar, Z., Kucuk, M., Dogan, H. (2017). A new colorimetric DPPH¢scavenging activity method with no need for a spectrophotometer applied on synthetic and natural antioxidants and medicinal herbs. Journal of Enzyme Inhibition and Medicinal Chemistry, 32(1), 640-647. DOI:10.1080/14756366.2017.1284068
Alara, O. R., Abdurahman, N. H., & Ukaegbu, C. I. (2018). Soxhlet extraction of phenolic compounds from Vernonia cinerea leaves and its antioxidant activity. Journal of Applied Research on Medicinal and Aromatic Plants, 11, 12“17. DOI:10.1016/j.jarmap.2018.07.003
Alara, O.R., Nour, A.H., Mudalip, S.K.A. (2019). Screening of microwave-assisted-batch extraction parameters for recovering total phenolic and flavonoid contents from Chromolaena odorata leaves through two-level factorial design. Indonesian Journal of Chemistry, 19(2), 511-521. DOI:10.22146/ijc.40863
Ghasemzadeh, A., Ghasemzadeh, N. (2011). Flavanoid and Phenolic acids: role and biochemical activity in plants and human. Journal of Medicinal Plants Research, 5(31), 6697-6703. DOI:10.5897/JMPR11.1404
Gul, R., Jan, S.U., Fardullah, S., Sherani, S., Jahan, Nusrat. (2017). Preliminary phytochemical screening, quantitative analysis of alkaloids, and antioxidant activity of crude plant extracts from Ephedra intermedia Indigenous to balochistan. Hindawi The Scientific World Journal, 1-7. DOI:10.1155/2017/5873648
Gurning, K. (2020). Determination antioxidant activities methanol extracts of bangun-bangun (Coleus amboinicus L.) leaves with DPPH method. Jurnal Pendidikan Kimia, 12(2), 62-69. DOI:10.24114/jpkim.v12i2.19397
Gurning, K., Boangmanalu, R., Simanjuntak, H.A., Singarimbun, N., Rahmiati, R., Lestari, W. (2020). Identification of secondary metabolites and antidiarrheal activity of pirdot leaves ethanol extract (Saurauia vulcani Korth.) from West Pakpak, North Sumatera Province, Indonesia. Rasayan Journal of Chemistry, 13(4), 2385-2389. DOI:10.31788/ RJC.2020.1345984
Gurning, K., Simanjuntak, H. A., Purba, H., Situmorang, R. F. R., Barus, L., & Silaban, S. (2021). Determination of total tannins and antibacterial activities ethanol extraction seri (Muntingia calabura L.) Leaves. Journal of Physics: Conference Series, 1811 012121. DOI:10.1088/1742-6596/1811/1/012121
Gurning, K., Haryadi, W., Sastrohamidjojo, H. (2021). Isolation and charactericzation of antioxidant compounds of bangun-bangun (Coleus amboinicus, L.) leaves from Nort Sumatera, Indonesia. Rasayan Journal of Chemistry, 14(1), 248-253. DOI: 10.31788/ RJC.2021.1416077
Gurning, K. (2020). Identification of secendary metabolic and test of activity ethyl acetate fraction of bangun-bangun (Coleus amboinicus Lour.) leaves as antioxidant. Biolink, 7(1), 117-122. DOI:10.31289/biolink.v7i1.3732
Haro, G., Iksen, I., Rumanti, R.M., Marbun, N., Sari, R.P., & Gultom, R.P.J. (2018). Evaluation of antioxidant activity and minerals value from watercress (Nasturtium officinale R.Br.). Rasayan Journal of Chemistry, 11(1), 232-237. DOI:10.7324/RJC.2018.1112011
Kumar, N., Goel, N. (2019). Phenolic acids: Natural versatile molecules with promising therapeutic applications. Biotecnology Reports, 24, 1-10. DOI:10.1016/j.btre.2019.e00370
Marjoni, M.R., Zulfisa, A. (2017). Antioxidant activity of methanol extract/fractions of senggani leaves (Melastoma candidum D. Don). Pharmaceutica Analytica Acta, 8(8), 1-6. DOI:10.4172/2153-2435.1000557
Rydlewski, A.A., Morais, D.R.d., Rotta, E.M., Claus, T., Vagula, J.M., Silva, M.C.d., Junior, O.O.S., & Visentainer, J.V. (2017). Bioactive compounds, antioxidant capacity, and fatty acids in different parts of four unexplored fruits. Journal of Food Quality, 1-9. DOI:10.1155/2017/8401074
Shahidi, F., Ambigaipalan, P. (2015). Phenolic and polyphenolics in food, beverages and spices: Antioxidant activity and health effects “ A review. Journal of Fuctional Foods, 18, 820-897. DOI:10.1016/j.jff.2015.06.018
Sirivibulkovit, K., Nouanthavong, S., Sameenoi, Y. (2018). Paper-based DPPH Assay for antioxidant activity analysis. Analytical Sciences, 34(7), 795-800. DOI:10.2116/analsci.18P014
Sundari, H., Prbowo, M.H., Racmawaty, F.J., Tamhid, H.A. (2015). Standardization of leaf extract of red betel (Piper crocatum) leaves using ethanol. Jurnal Kedokteran dan Kesehatan Indonesia, 7(1), 3-9. DOI:10.20885/JKKI.Vol7.Iss1.art2
Wojcik, M., Burzynska-Pedziwiatr, I., & Wozniak, L.A. (2010). A Review of natural and synthetic antioxidants important for health and longevity. Current Medicinal Chemistry, 17(28), 3262-3288. DOI: 10.2174/092986710792231950
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