CHARACTERISTICS OF ELECTRODE MEMBRANE PVA-ENZIME COATING PVC-PLASTICIZER KTpClPB FOR UREA BIOSENSOR
DOI:
https://doi.org/10.24114/jltp.v1i2.22713Keywords:
Electrode Selective Ion (ISE), Potentiometric, PVA-Enzyme, PVC-Plastisizer KTpClPBAbstract
This study aims to determine the effect of the composition comparison of the PVA-Enzyme urease coating PVC-Plastisizer KTpClPB membrane with memmbran characterization using FTIR and analyze membrane electrodes with XRD. The method used is the potentiometric method using the Electrode Selective Ion (ISE), where the polymer used is PVA and PVC as a coating with a composition ratio of PVA: PVC is 1: 1. The same PVA and PVC were used, namely 0.0350 g, with 2 mg of enzyme and 0.0500 g and 0.0120 g of KTpClPB plasticizer. The best composition result is the PVA-Enzyme PVC-Plastisizer coating enzyme KTpClPB 0.0500 g membrane electrode characterization using XRD shows the highest intensity value is at an angle of 2θ = 44.020 which is 1,626 count second (cts) and membrane characterization using FTIR shows that there is a functional group OH, CH, C≡C, NH, CO and CH. The addition of PVA-Enzyme coating will reduce or even eliminate the amount of Wolframm and can reduce the amorphous properties of the membrane electrodes.References
Arora, N. (2013). Recent Advances in Biosensors Technology: A Review. Octa Journal of Biosciences, 1 (2) : 147-150.
Corcuera, D. R. J., & Cavalieri, P. R. (2003). Biosensor. Encyclopedia of Agricultural, Food, and Biological Engineering.
Fauziyah, B. (2012). Optimasi Parameter Analitik Biosensor Urea Berbasis Immobilisasi Urease dalam Membran Polianilin. Saintis, 1 (1) : 65-72.
Goncalves, A. M., Pedro, A. Q., Santos, F. M., Martins, L. M., Maia, C. J., Queiroz, J. A., & Passarinha, L. A. (2014). Trends in Protein-Based Biosensor Assemblies for Drug Screening and Pharmaceutical Kinetic Studies. Molecules,www.mdpi.com/journal 19.
Gupta, B., Singh, S., Mohan, S., & Prakash R. (2010). Urea Biosensor based on Conducting Polymer Transducers. Biosensor. India, Intech.55
Hakim S, A., Tarigan, K., Sebayang, K., Sembiring, T., & Sitomurang, M. (2017). The Characteristic Mebrane PVA-Enzim and Coating PVC-Plastisizer with XRD. The 4th Annual International Seminar on Trends in Science and Science Education State University of Medan 32-40
Hakim S, A., Tarigan, K., Sebayang, K., Sembiring, T., & Sitomurang, M. (2018). The Characteristic Mebrane PVA-Enzim and Coating PVC-Plastisizer with SEM-EDX. International Journal of Scientific & Engineering Research,9(1) : 1229-1236.
Hakim S, A., Tarigan, K., Sitomurang, M., & Sembiring, T. (2018). Synthesis of Urea Sensors using Potentiometric Methods with Modification of Electrode Membranes Indicators of ISE from PVA-Enzymes Coating PVC-KTpClPB. The 8th International Conference on Theoretical Apllied Physics, 1-14.
Hakim S, A., Sembiring, T., Tarigan, K., Sebayang, K., Situmorang, M., & Noer, N. M. (2019). Characterization of Membrane PVA-Enzyme Coating PVC-KTpClPB as Urea Sensor with Potentiometric Method. RASAYAN J. Chem,12 (2) : 780-786.
Kale, P. D., Bodade, A. B., & Chaudhari, G. N. (2016). Study of Immobilization of Urease on PVA-Nano NiFe2O4 Nanocomposite for Biosensor Application. International Journal of Pharmacy and Pharmaceutical Science, 8 (7) : 380-385.
Rohaeti, E. (2009). Karakterisasi Biodegradasi Polimer. Prosiding Seminar Nasional Penelitian dan Penerapan MIPA, UNY, 248-257.
Tamba, D. (2016). Immobilisasi Enzim Urease untuk Pembuatan Sensor Kimia dalam Penentuan Urea. Skripsi, FMIPA, UNIMED, Medan.
Yulianti, E, Saputri R. D., Sudaryanto, Jodi, H., & Salam, R. (2013). Pembuatan Bahan polimer Elektrolit Padat Berbasis Nanokomposit Kitosan Montmorillonite untuk Aplikasi Baterai, Jurnal Kimia Kemasan 35 (2) ; 77-83
Downloads
Published
Issue
Section
License
Copyright (c) 2021 Journal of Learning and Technology in Physics
This work is licensed under a Creative Commons Attribution 4.0 International License.
