FORMULATION AND TESTING OF ANTICONVULSANT ACTIVITY OF TITANUS LEAVES (Leea aequanta L.) ETHANOLIC EXTRACT CAPSULES ON GUINEA PIG (Cavia cobaya) TRACHEA SMOOTH MUSCLE IN VITRO

Authors

  • Dian Ika Perbina Meliala Institut Kesehatan Deli Husada Deli Tua https://orcid.org/0000-0002-0646-686X
  • Sofia Rahmi Jurusan Farmasi, Progam Studi Farmasi, Fakultas Farmasi, Institut Kesehatan Deli Husada, Deli Tua
  • Delisma Simorangkir Simorangkir Jurusan Farmasi, Progam Studi Farmasi, Fakultas Farmasi, Institut Kesehatan Deli Husada, Deli Tua
  • Beriman Putra Harefa Jurusan Farmasi, Progam Studi Farmasi, Fakultas Farmasi, Institut Kesehatan Deli Husada, Deli Tua

DOI:

https://doi.org/10.24114/jbio.v7i3.28388

Keywords:

Capsule preparation, EEDT, guinea pig trachea, relaxation, in vitro.

Abstract

The main obstacle to consuming traditional medicine is the compounding process which is considered troublesome so that it often causes discomfort to consume it. For the purpose of efficiency, easy and practical in use, need to be made in dosage forms such as capsules. Capsules are solid preparations consisting of a drug in a soluble hard or soft shell. Titanus leaf (Leea aequata L.) is a medicinal plant that is used by the people of the Tanah Karo area, North Sumatra Province as an antitetanus drug. Tetanus is an acute and often fatal disease caused by an exotoxin produced by the bacterium Clostridium tetani. Tetanus often begins with a mild spasm of the jaw muscles which is often referred to as trismus. Sometimes the spasm affects the muscles that help with breathing, which can cause breathing problems. The aim of this study was to formulate EEDT capsules and to test its anticonvulsant activity on the smooth muscle of the guinea pig trachea in vitro. The relaxation effect was tested after the guinea pig's trachea was contracted with acetylcholine (EC80 = 1,0454 x 10-3 M), then the cumulative concentration of EEDT capsules was 0.5-4 mg/mL and atropine sulfate concentration of 6.95 x 10-6 - 2.08 x 10-2 mg/mL. The results of the relaxation effect test showed that EEDT granules could reduce acetylcholine-induced guinea pig trachea smooth muscle contraction. EEDT granules at a concentration of 3.5 mg/mL (102,8817 ± 0,1316) had the same ability as atropine sulfate 6.95 x 10-3 mg/mL (102,7748 ± 0,3231) in reducing guinea pig trachea smooth muscle contraction induced by acetylcholine 1,0454 x 10-3 M (p>0.005). EEDT capsules with a concentration of 3.5 mg and 4 mg had the same effectiveness, that is 102,8817 ± 0,1316. From this study, it can be concluded that the EEDT capsule preparation has a relaxing effect on the smooth muscle of the isolated guinea pig trachea with an ability that is not much different from atropine sulfate.

Author Biography

Dian Ika Perbina Meliala, Institut Kesehatan Deli Husada Deli Tua

Departemen Kimia Farmasi

References

Atkinson, W. (2012). Tetanus Epidemiology and Prevention of Vaccine-Preventable Diseases (12 ed.). Public Health Foundation. Hal. 291“300.

Augsburger, L.L. (2000). Modern Pharmaceutics: Hard and soft Gelatin Capsule. (Ed. 2). New York: Mercel Dekker. Halaman 212.

BPOM RI. (2012). Atropin Sulfat. Jakarta: Sentra Informasi Keracunan Nasional (Sikernas). Hal 95.

Ditjen POM. (1995). Farmakope Indonesia. Edisi keempat. Jakarta: Departemen Kesehatan RI. Halaman 5, 1035.

Khare, C.P. (2007). Indian Medicinal Plants. New Delhi: Springer Science Business Media, LCC. Hal. 366.

Sinaga, E. (2018). Uji Aktivitas Antikejang Ekstrak Etanol Daun Titanus (Leea Aequata L.) Terhadap Ileum Marmut Terpisah (Cavia Porcellus) Secara In Vitro. TM Conference Series 01, 1, 320-330.

Soediono, J. B., dkk. (2019). Uji Skrining Fitokimia dan Evaluasi Sediaan Salep Ekstrak Etanol Daun Kemangi (Ocimum sanctum (L.)) Dengan Menggunakan Basis Salep Hidrokarbon dan Basis Salep Serap. Jurnal Polanku. Volume 1. Nomor 1. ISSN: 2656-7733. Halaman 26-27.

Suharmiati, Handayani, L. (2006). Cara Benar Meracik Obat Tradisional. Cetakan Pertama. Jakarta : Agromedia Pustaka. Halaman 1.

Vogel, H.G., Bernward, A.S., Jurgen, S., Gunter, M., dan Wolfgang, F.V. (2002). Drug Discovery and Evaluation: Pharmacological Assays, Spinger-Verlag, Berlin Heidelberg, Germany. Hal. 98.

Voight, R. (1995). Buku Pelajaran Teknologi Farmasi. Cetakam II. Penerjemah: Soedani N. S. UGM-Press. Yogyakarta. Halaman 159.

Downloads

Published

2022-01-03

Issue

Vol. 7 No. 3 (2021): Jurnal Biosains

Section

Article

License

Copyright (c) 2022 Dian Ika Perbina Meliala

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

For Authors Jurnal Biosains agree to the following terms:

Authors retain copyright and grant the Jurnal Biosains (JBIO) right of first publication with the work simultaneously licensed under a Creative Commons Attribution License (CC BY-SA 4.0) that allows others to share (copy and redistribute the material in any medium or format) and adapt (remix, transform, and build upon the material) the work for any purpose, even commercially with an acknowledgement of the work's authorship and initial publication in JBIO (Jurnal Biosains)

Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in JBIO (Jurnal Biosains)

Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).