Exploration of extraceluller enzymes-producing bacteria symbiont Sponge Phorbas sp. from Nngge Island, Sibolga

Authors

  • Endang Sulistyarini Gultom Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Medan, Medan 20221, Indonesia
  • Sarah Gladies Aurelia Sijabat Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Medan, Medan 20221, Indonesia
  • Ulfayani Mayasari Department of Biology , Faculty of Science and Technology, UIN Sumatera Utara, Medan, Indonesia

DOI:

https://doi.org/10.24114/jmns.v1i1.28470

Abstract

This study aims to determine the bacteria that is in symbiont sponges and has the potential to produce extracellular enzymes such as protease, amylase, cellulase and lipase. The sea sponge obtained from Ngge Island, Sibolga is a type of Phorbas sp. The enzymatic activity test was carried out. Bacterial isolates obtained from the sponge Phorbas sp. there are 7 bacterial isolates. Seven bacterial isolates had the ability to produce extracellular enzymes and proteolytic, amylolytic, lipolytic and cellulolytic bacteria. Proteolytic activity using test media enriched with skim milk (1%), amylolytic activity using starch-enriched test media (1%), cellulolytic activity using CMC enriched test media (1%) and lipolytic activity using Tween 80 enriched test media (1%). Based on the results of the research, seven isolates of sponge symbiont bacteria have enzymatic activity of amylase and cellulase, namely bacterial isolates P1, P2, P3, P4, P5, P6, P7 and six (6) bacterial isolates which have protease and lipase enzymatic activity are bacterial isolates P1, P2, P3, P4, P6, P7.

References

Aiyer, P.V. (2005) Review: Amiylases and their applications. African J. Biotechnol., 4(13): 1525-1529

Feby, A., Nair, S. (2010) Sponge-associated bacteria of Lakshadweep coral reefs, India: resource for extracellular hydrolytic enzymes. Adv. Biosci. Biotechnol., 1: 330“337

Gultom, E.S., Suryanto, D., Munir, E., Diningrat, D.S. (2017) Bacteria extract activity associated with sponge Haliclona sp.2 and Axinellid sp. as antibacterial. Int. J. Adv. Res., 5(1): 751-759

Marzuki, I., Noor, A., Nafie, N.L., Djide, M.N. (2014) Isolasi dan identifikasi bakteri Shimbion Spons penghasil enzim amilase asal pantai Melawai Balikpapan. J. Ilmiah œdr. Aloei Saboe, 1(2): 11“18

Moat, A.G., Foster, J.W., Spector, M.P. (2002) Microbial physiology fourth edition. New York: Wiley-liss, Inc.

Muniarsih, T., Rasyid, A. (2010) Potensi bakteri yang berasosiasi dengan spons asal Barrang Lompo (Makassar) sebagai sumber bahan antibakteri. Oseanol. Limnol. Indonesia, 36(3): 281“292

Okoko, F.J., Ogbomo, O. (2010) Amylolytic properties of fungi associated with spoilage in bread. Continent. J. Microbiol., 4: 1“7

Restuati, M., Gultom, E.S. (2012) Uji potensi bakteri yang berasosiasi dengan spons asal Pulau Ngge (Sibolga) sebagai sumber antibakteri. J.l Saintika, 12(2): 98“104

Riffiani, R., Sulistinah, N. (2010) Penapisan mikroba laut perombak senyawa nitril dan protein yang diisolasi dari spons di Perairan Ternate. J. Biol. Indonesia, 6(3): 353“365

Setyati, W.A., Subagiyo, S. (2012) Isolasi dan seleksi bakteri penghasil enzim ekstraseluler (proteolitik, amilolitik, lipolitik, dan selulolitik) yang berasal dari sedimen kawasan mangrove. J. Ilmu Kelautan, 17(3): 164“168

Setyati, W.A., Habibi, A.S., Subagiyo, S., Ridlo, A., Nirwani, N., Pramesti, R. (2016) Skrining dan seleksi bakteri simbion spons penghasil enzim ekstraseluler sebagai agen bioremediasi bahan organik dan biokontrol vibriosis pada budidaya udang. J. Kelautan Tropis, 19(1): 11“20

Shanmughapriya, S., Krishnaveni, Joseph S., Gandhimathi, R., Arunkumar, M., Thangavelu, T., Kiran, G.S., Natarajaseenivasan, K. (2008) Optimization of extracellular thermotolerant alkaline protease produced by marine Roseobacter sp. (MMD040). Biopro. Biosyst. Eng., 31: 427“433

Zhang, C., Kim, S. (2010) Research and application of marine microbial enzymes: status and prospects. Mar. Drugs, 8(6): 1920“1934

Downloads

Published

2021-10-08

Issue

Section

Articles